Editors: Najam A. Sharif, Sunny E. Ohia

Research Protocols for Ophthalmic Disease Mechanisms and Therapeutics: Glaucoma - Ocular Hypertension

eBook: US $129 Special Offer (PDF + Printed Copy): US $207
Printed Copy: US $142
Library License: US $516
ISBN: 978-981-5324-34-1 (Print)
ISBN: 978-981-5324-33-4 (Online)
Year of Publication: 2025
DOI: 10.2174/97898153243341250101

Introduction

Research Protocols for Ophthalmic Disease Mechanisms and Therapeutics: Glaucoma – Ocular Hypertension offers a detailed, step-by-step protocols for isolating, characterizing, and analyzing cells and tissues of the eye’s anterior segment—where OHT originates—using techniques from molecular biology, pharmacology, and histology.

Across eight thematic sections, the book covers ocular anatomy, drug screening, receptor localization, cell-based assays, in vitro and in vivo models, drug formulation, and cutting-edge technologies such as gene editing, single-cell RNA sequencing, and stem cell therapies. Each protocol is designed to support reproducibility and translational relevance.

Key Features:

  • - Details validated protocols for drug discovery and disease modeling
  • - Demonstrates methods for studying receptor function and pharmacokinetics
  • - Provides animal models and ex vivo systems to evaluate therapeutic strategies
  • - Incorporates emerging tools such as CRISPR, metabolomics, and exosomes
  • - Bridges experimental design with clinical relevance for glaucoma research


Readership:

Researchers, clinicians, and industry experts working in ophthalmic drug development, vision science, and translational eye disease research.

Contributors

Editor(s):
Najam A. Sharif
Vice President and Head of Research & Development
Nanoscope Therapeutics Inc. 2777 N. Stemmons Fwy
Suite 1102, Dallas
TX-75207, USA


Sunny E. Ohia
Department of Pharmaceutical Sciences
College of Pharmacy and Health Sciences
Texas Southern University
Houston, Texas
USA




Contributor(s):
Anthonia Okolie
Department of Pharmaceutical Sciences
College of Pharmacy and Health Sciences, Texas Southern University
Houston, Texas
USA


Amy Chen
Department of Ophthalmology
Novartis Institute of Biomedical Research
22 Windsor St. Cambridge, MA-02452
USA


Andrea M. Unser
Colleges of Nanoscale Science and Engineering
SUNY Polytechnic Institute
257 Fuller Road, Albany 12203, New York
USA


Aparna Rao
Hyderabad Eye Research Foundation, , ,
LV Prasad Eye Institute, Patia, Bhubaneswar, MTC Campus
Bhubaneswar, Odisha
India


Alyssa Hostert
Department of Pharmacy Sciences
School of Pharmacy and Health Professions, Creighton University
Omaha, Nebraska
USA


Anna Van Koeverden
Department of Optometry & Vision
202 Berkeley St, Parkville, The University of Melbourne
Victoria 3010
Australia


Abdelrahman Anter
Bascom Palmer Eye Institute
University of Miami Miller School of Medicine
Miami, FL 33136
USA


Adnan Dibas
Nanoscope Technologies
1312 Brown Trail
Bedford, TX 76022
USA


Alex Leung
UCL Institute of Ophthalmology
University College London
London
United Kingdom


Anna Mueller
Bascom Palmer Eye Institute
University of Miami Miller School of Medicine
Miami, FL 33136
USA


Brianna Caroline Afiat
Department of Optometry & Vision
202 Berkeley St, Parkville, The University of Melbourne
Victoria 3010
Australia


Bang Viet Bui
Department of Optometry & Vision
202 Berkeley St, Parkville, The University of Melbourne
Victoria 3010
Australia


Betsy Benitez
Miami Integrative Metabolomics Research Center
Miami, FL 33136
USA


Catherine A. Opere
Department of Pharmacy Sciences, School of Pharmacy and Health Professions
Creighton University
Omaha, Nebraska
USA


Christopher W. Wilson
Department of Ophthalmology
Novartis Institute of Biomedical Research
22 Windsor St. Cambridge, MA-02452
USA


Christine Tram Oanh Nguyen
Department of Optometry & Vision
202 Berkeley St, Parkville, The University of Melbourne
Victoria 3010
Australia


Carol B. Toris
Havener Eye Institute, Department of Ophthalmology and Visual Sciences
The Ohio State University Wexner Medical Center
915 Olentangy River Rd, Suite 3010B, Columbus, OH 43212
USA


Charles E. Amankwa
Department of Pharmacology and Neuroscience and The North Texas Eye Research Institute
University of North Texas Health Science Center
Fort Worth, TX 76107
USA


Dorette Z. Ellis
Department of Pharmaceutical Sciences
University of North Texas System College of Pharmacy, North Texas Eye Research Institute
University of North Texas Health Science Center, Fort Worth, Texas
USA


Da Zhao
Department of Optometry & Vision
202 Berkeley St, Parkville, The University of Melbourne
Victoria 3010
Australia


Dharmalingam Kuppamuthu
Department of Biotechnology
Aravind Medical Research Foundation - Affiliated to Alagappa University
Karaikudi, Tamil Nadu
India
/
Department of Proteomics
Aravind Medical Research Foundation
Madurai, Tamil Nadu
India


Fatima Muili
Department of Pharmaceutical Sciences
College of Pharmacy and Health Sciences, Texas Southern University
Houston, Texas
USA


Fatima Shirazee
Department of Pathology and Molecular Medicine
McMaster University
Hamilton
Canada


Ganesh Prasanna
Department of Ophthalmology
Novartis Institute of Biomedical Research
22 Windsor St. Cambridge, MA-02452
USA


Gowri Priya Chidambaranathan
Department of Immunology and Stem Cell Biology
Aravind Medical Research Foundation
Madurai, Tamil Nadu
India
/
Department of Biotechnology
Aravind Medical Research Foundation - Affiliated to Alagappa University
Karaikudi, Tamil Nadu
India


Gulab Zode
Department of Pharmacology and Neuroscience and The North Texas Eye Research Institute
University of North Texas Health Science Center
Fort Worth, TX 76107
USA


Hanna Rao
Department of Arts and Science
McMaster University
Hamilton, Ontario
Canada


Iswarya Radhakrishnan
Department of Immunology and Stem Cell Biology
Aravind Medical Research Foundation
Madurai, Tamil Nadu
India
/
Department of Biotechnology
Aravind Medical Research Foundation - Affiliated to Alagappa University
Karaikudi, Tamil Nadu
India


Isabella Moceri
Miami Integrative Metabolomics Research Center
Miami, FL, 33136
USA


J. Cameron Millar
North Texas Eye Research Institute (NTERI), Department of Pharmacology & Neuroscience
University of North Texas Health Science Center (UNTHSC)
3500 Camp Bowie Boulevard, Room IREB-511, Fort Worth, TX 76107
USA


Judith West-Mays
Department of Pathology and Molecular Medicine
McMaster University
Hamilton
Canada


James E. Chastain
Novartis Institutes for BioMedical Research (Retired)
The Woodlands, 26 Dawning Flower Dr., The Woodlands
TX 77375
USA


Jack Hopkins
UCL Institute of Ophthalmology
University College London
London
United Kingdom


Karen Y. Torrejon
Colleges of Nanoscale Science and Engineering
SUNY Polytechnic Institute
257 Fuller Road, Albany 12203, New York
USA


Kalu K. Ngele
Department of Pharmaceutical Sciences
College of Pharmacy and Health Sciences, Texas Southern University
Houston, Texas
USA


Krishnadas Subbaiah
Glaucoma Services
Aravind Eye Hospital and Post Graduate Institute of Ophthalmology
Madurai, Tamil Nadu
India


Len Pinchuk
Research & Development
InnFocus Inc. (A Santen Co.)
12415 S.W. 136 Ave., Unit 3, Miami, FL 33186
USA


Mary J. Kelley
Department of Ophthalmology
Casey Eye Institute Oregon Health & Science University Portland
Oregon
USA


Mohammad Shahidullah
Department of Physiology and Department of Ophthalmology & Vision Science
College of Medicine, University of Arizona
1501 N Campbell Avenue, Tucson, AZ 85724
USA


Munmun Chakraborthy
Hyderabad Eye Research Foundation, , ,
LV Prasad Eye Institute, Patia, Bhubaneswar, MTC Campus
Bhubaneswar, Odisha
India


Muthukkaruppan Veerappan
Department of Immunology and Stem Cell Biology
Aravind Medical Research Foundation
Madurai, Tamil Nadu
India


Najam A. Sharif
Vice President and Head of Research & Development Nanoscope Therapeutics Inc.
2777 N. Stemmons Fwy, Suite 1102
Dallas, TX-75207
USA


Nozhat Choudry
Santen Inc., 6401 Hollis Street
Suite 125, Emeryville
CA-94608
USA


Nicholas Delamere
Department of Physiology and Department of Ophthalmology & Vision Science
College of Medicine, University of Arizona
1501 N Campbell Avenue, Tucson, AZ 85724
USA


Onkar N. Singh
CMC/Manufacturing/Technical Operations
Alentia Therapeutics Inc., Fort Worth, 2648 Gosling Way
Fort Worth, TX-76118
USA


Peter G. Klimko
Novartis Pharmaceuticals Corporation
Fort Worth
TX 76107
USA


Pujhitha Ramesh
Colleges of Nanoscale Science and Engineering
SUNY Polytechnic Institute
257 Fuller Road, Albany 12203, New York
USA


Pratik Bhankele
Department of Pharmacy Sciences
School of Pharmacy and Health Professions, Creighton University
Omaha, Nebraska
USA


Pei Ying Lee
Department of Optometry & Vision
202 Berkeley St, Parkville, The University of Melbourne
Victoria 3010
Australia


Rachel N. Andrews
Therapeutic Area Lead for Ophthalmology
Safety Assessment – Toxicology, Genentech Research and Development
A Member of the Roche Group, 1 DNA Way, MS# 441A, South San Francisco, CA 94080
USA


Raj Patil
OKYO Pharma Ltd
420 Lexington Ave, Suite 1402
New York, NY-10170
USA


Sunny E. Ohia
Department of Pharmaceutical Sciences
College of Pharmacy and Health Sciences, Texas Southern University
Houston, Texas
USA


Saima Chaudhry
Univ N. Texas at Arlington
701 S Nedderman Dr, Arlington
TX-76019
USA


Suchismita Acharya
AyuVis Research Inc.
3500 Camp Bowie Blvd, EAD #116J
Fort Worth, TX 76107
USA
/
Department of Pharmacology & Neuroscience
North Texas Eye Research Institute
Fort Worth, TX 76107
USA


Shan Fan
Department of Ophthalmology and Visual Sciences
University of Nebraska Medical Center
Omaha, Nebraska
USA


Sanjoy K. Bhattacharya
University of Miami Miller School of Medicine
1638 NW 10th Avenue
Suite 707Am Miami, FL 33136
USA


Shahid Husain
Department of Ophthalmology
Storm Eye Institute, Medical University of South Carolina
167 Ashley Ave, Charleston, SC-29425
USA


Samarendra Mohanty
Nanoscope Technologies
1312 Brown Trail
Bedford, TX 76022
USA


Schuichi Koizumi
Department of Neuropharmacology
Interdisciplinary Graduate School of Medicine & GLIA Center
University of Yamanashi, Yamanashi
Japan


Sam Yacoub
Department of Pharmacology and Neuroscience and The North Texas Eye Research Institute
University of North Texas Health Science Center
Fort Worth, TX 76107
USA


Shin-ichi Ohnuma
UCL Institute of Ophthalmology
University College London
London
United Kingdom


Ted S. Acott
Department of Ophthalmology
Casey Eye Institute Oregon Health & Science University Portland
Oregon
USA


Thomas R. Vollmer
Department of Ophthalmology
Novartis Institute of Biomedical Research
22 Windsor St. Cambridge, MA-02452
USA


Ursula Schlötzer-Schrehardt
Department of Ophthalmology
Friedrich-Alexander University Erlangen-Nürnberg
Schwabachanlage 6, 91054 Erlangen
Germany


Veluchamy A. Barathi
Translational Pre-Clinical Model Platform, Singapore Eye Research Institute, ACP in Ophthalmology & Visual Sciences
DUKE-NUS Graduate Medical School, Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore
Singapore 119077
Singapore


VijayKrishna Raghunathan
Department of Ophthalmology
Novartis Institutes for BioMedical Research
22 Windsor St, Cambridge, MA 02139
USA


Vickie Hoi Ying Wong
Department of Optometry & Vision
202 Berkeley St, Parkville, The University of Melbourne
Victoria 3010
Australia


Vladimir Bantseev
Therapeutic Area Lead for Ophthalmology
Safety Assessment – Toxicology, Genentech Research and Development
A Member of the Roche Group, 1 DNA Way, MS# 441A, South San Francisco, CA 94080
USA


Wei Zhang
North Texas Eye Research Institute (NTERI), Department of Pharmacology & Neuroscience
University of North Texas Health Science Center (UNTHSC)
3500 Camp Bowie Boulevard, Room IREB-511, Fort Worth, TX 76107
USA


Ya Fatou Njie-Mbye
Department of Pharmaceutical Sciences
College of Pharmacy and Health Sciences, Texas Southern University
Houston, Texas
USA


Yasushi Kato
Research & Development
InnFocus Inc. (A Santen Co.)
12415 S.W. 136 Ave., Unit 3, Miami, FL 33186
USA


Youichi Shinozaki
Department of Neuropharmacology
Interdisciplinary Graduate School of Medicine & GLIA Center
University of Yamanashi, Yamanashi
Japan
/
Visual Research Project
Tokyo Metropolitan Institute of Medical Science
Tokyo
Japan


Zheng He
Department of Optometry & Vision
202 Berkeley St, Parkville, The University of Melbourne
Victoria 3010
Australia




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