Authors: Ishfaq Ahmad Rather, Rashid Ali

Indicator Displacement Assays (IDAs): An Innovative Molecular Sensing Approach

eBook: US $49 Special Offer (PDF + Printed Copy): US $84
Printed Copy: US $59
Library License: US $196
ISBN: 978-981-5165-92-0 (Print)
ISBN: 978-981-5165-91-3 (Online)
Year of Publication: 2024
DOI: 10.2174/97898151659131240101

Introduction

This book explores a revolutionary sensing technique called Indicator Displacement Assays (IDAs). It is designed to provide readers with a comprehensive understanding of the conceptual foundation and wide-ranging applications of IDAs. The book aims to fulfill a crucial gap in the existing references on the subject.

The content starts by explaining fundamental concepts, design strategies, and the scope of IDAs. Subsequent chapters elaborate on the intricate molecular recognition of various analytes, offering insights into sensing mechanisms for cationic, anionic, and neutral molecules. The design and construction of colorimetric, fluorescence, and metal complexing IDAs are also thoroughly explored in later chapters, followed by recent extensions of IDAs, including enantioselective indicator displacement assays, intramolecular indicator displacement assays, reaction-based indicator displacement assays, and more. Innovative applications of IDAs, such as sensory arrays and electrochemical sensors, are also discussed in detail, providing a comprehensive understanding of their conceptual foundations and practical implementations.

The book is a primer on IDAs for researchers who want to understand the fundamentals of the technique, as well as postgraduate students aiming to specialize in supramolecular and analytical chemistry.

Readership:

Chemistry (Supramolecular chemistry, analytical chemistry, material sciences and technology, pharmaceutical chemistry, inorganic chemistry and organic chemistry), pharmaceutical and life sciences researchers; laboratory technicians.

Preface

Among diverse sensing assays, the indicator displacement assay (IDA)-based competitive experiments employing various indicators are most acknowledged in supramolecular analytical chemistry. IDA experiments have shown a promising role in exploring the interactions between biological hosts ( e.g., proteins, nucleic acids, etc.) and various small molecules of interest, which are otherwise difficult to achieve through other sensing methodologies. This particular competitive technique has great potential to accomplish a rapid analysis of desired analytes because of being easily adaptable for different receptors.

In the first chapter of the book “Indicator Displacement Assays (IDAs): An Innovative Molecular Sensing Approach”, we have assembled the background, conceptual details, design, and construction strategies, in addition to the need and urgency to replace indicator spacer receptor (ISR) approach with IDA having numerous advantages. The second, third, and fourth chapter, besides dealing with the molecular recognition of various analytes (cationic, anionic, and neutral), also deal with the design and construction of colorimetric, fluorescence, and metal complexing IDAs, respectively. The fifth chapter deals with various aspects of recent extensions of IDAs like enantioselective indicator displacement assays (e-IDAs), intramolecular indicator displacement assays (I-IDAs), reaction-based indicator displacement assays (R-IDAs), mechanically controlled indicator displacement assays (MC-IDAs), allosteric indicator displacement assays (A-IDAs), dimer dye assembly assay (DDA), and quencher displacement assay (QDA). The sixth chapter discusses conceptual details and various applications of IDA-based sensory arrays. The last chapter of this book deals with the design, fabrication, and applications of IDA-based electrochemical sensors.

This book strives to collectively present the design, construction, and molecular recognition characteristics of IDA-based sensors along with recent advancements, which in turn will provide newer insights to readers as well as young researchers who are willing to work in the field of sensory materials.

Ishfaq Ahmad Rather
Organic and Supramolecular Functional Materials Research Laboratory
Department of Chemistry
Jamia Millia Islamia, Jamia Nagar, Okhla, New Delhi-110025,
India

&

Rashid Ali
Organic and Supramolecular Functional Materials Research Laboratory
Department of Chemistry
Jamia Millia Islamia, Jamia Nagar, Okhla, New Delhi-110025,
India